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Saturday, May 19
The BD Cytometer Set-up and Tracking
(CST) System: A New Paradigm for a Multi-Color, Digital World
BD Biosciences | 10975 Torreyana Road | San Diego, CA 92121 | Phone:
877 232-8995; Fax: 858 812-8888; Email:
techserv@bd.com |
www.bdbiosciences.com
12 Noon – 1:00 P.M. – Room A110/111
Presenter: Alan Stall, Ph.D., Director, Advanced Cytometry Technologies,
BD Biosciences
To take full advantage of modern flow cytometers
with capability of measuring up to 12-16 individual fluorescence
parameters, it is critical they be fully characterized, properly
optimized, set-up and standardized for day-to-day performance and
reproducibility. This seminar introduces the BD Cytometer Set-up &
Tracking (CST) system, which automatically performs these functions on
BD digital flow cytometers. It covers the theory and practice of
measuring critical cytometer performance parameters including: Detector
Linearity; Fluorescence Detector Efficiency (Q); Light Background (B);
Electronic Noise; and fluorescence Coefficient of Variation (CV). We
will present how these performance measurements are used to optimize PMT
voltages and maximize resolution sensitivity for multi-color
experiments. Finally we demonstrate how the CST system automates
reproducible daily Cytometer Set-up and tracks performance.
Discover MHC Class II Ultimers™, a
novel MHC multimer technology for the detection of antigen-specific CD4+
T cells
ProImmune Ltd | The Magdalen Centre | Oxford, Oxfordshire | Ox4
4GA. U.K. | Phone: +44 (0) 870 042 7279; Fax: +44 (0) 870 712 0588;
Email:
marketing@proimmmune.com |
www.proimmune.com
12 Noon – 1:00 P.M. – Room A108/109
Presenter: Jeremy Fry
MHC Class II Ultimers™, the result of several
years of intensive research at ProImmune, finally allow researchers
access to an affordable tool for evaluating and characterizing CD4+
single antigen-specific T cells. This seminar will describe how you can
apply this exciting new technology to your antigen-specific CD4+ T cell
research in applications including flow cytometry and magnetic bead
enrichment.
Sunday, May 20
amaxa GmbH | Nattermannallee 1 | Koeln,
NRW 50829, Germany | Phone: +4922199199400; Fax: +4922199199499; Email:
scientific-support@amaxa.com |
www.amaxa.com
8:00 A.M. – 9:00 A.M. – Room A108/109
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TIM-3 is a Negative
Regulator of Human Th1 Cells: Implications for Human Inflammatory
DiseasesPresenter: David E. Anderson, Center for Neurologic Diseases,
Brigham & Women’s Hospital and Harvard Medical School
Using two complementary approaches, blocking monoclonal antibodies and
siRNA technology, we will present data demonstrating that TIM-3 is a
negative regulator of human Th1 cells. We will also present data on the
relevance of the TIM-3 pathway to regulation of the human autoimmune
disease multiple sclerosis (MS) as well as immunity directed human brain
tumors.
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FoxP3 Enhances HIV-1
Gene Expression by Modulating the LTR Chromatin Structure and NFkB
Occupancy in Human T cells
Presenter: Derek Holmes, Department of Microbiology and Immunology,
Lineberger Comprehensive Cancer Center, University of North Carolina at
Chapel Hill
Regulatory T cell development and function is critically dependant on
the transcription factor FoxP3. FoxP3 represses IL-2 by inhibiting the
AP-1:NFAT interaction at the promoter and is associated with decreased
histone acetylation. We describe a mechanism wherein FoxP3 activates
HIV-1 LTR gene expression by modulating chromatin structure and
promoting NFκB occupancy.
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Nucleofection of
Primary Blood Cells and Difficult-to-Transfect Suspension Cell Lines
Presenter: Oliver Gresch, amaxa biosystems
Using the Nucleofector technology primary blood cells and suspension
cell lines can be transfected with high efficiencies. Here we present
latest news on DNA and siRNA transfections as well as results obtained
with the 96-well Shuttle System.
Phosphorylation-State Analysis in
Single Cells: Techniques and Applications
BD Biosciences | 10975 Torreyana Road | San Diego, CA 92121 |
Phone: 877 232-8995; Fax: 858 812-8888; Email:
techserv@bd.com |
www.bdbiosciences.com
12 Noon – 1:00 P.M. – Room A108/109
Presenter: Robert Balderas, VP of R&D, BD Biosciences
Intracellular assays of signaling systems have
been limited by the inability to correlate functional subsets of cells
in complex populations on the basis of active protein states within the
native context of the cell. We demonstrate the ability to simultaneously
monitor active protein states via phospho-epitope recognition in
subpopulations of complex cell populations by multiparameter flow-cytometric
analysis. Multi-dimensional assessment of active protein states, in
combination with surface marker and other flow cytometric detectable
parameters (i.e., cytokines, apoptosis), can provide functional
assessment on a single cell level that may have utility in clinical
diagnostics and/or disease progression. Furthermore, the ability to
profile both activating and inhibiting conditions of multiple protein
states simultaneously within the cell in a rapid and parallel manner may
be extended to pharmaceutical screening of compounds.
The Immune Epitope Database & Analysis Resource
La Jolla Institute for Allergy & Immunology | 9420 Athena Circle | La
Jolla, CA 92037 | Phone: 858 752-6541 | Fax: 858 752-6987 | Email:
contact@immuneepitope.org
| www.immuneepitope.org
1:00 P.M. – 3:00 P.M. – Room A110/111
Presenter: Bjoern Peters, Ph.D.
The Immune Epitope Database and Analysis Resource
(IEDB) is a freely available online resource supported by the National
Institutes of Health. The IEDB provides open access to published data
related to antibody and T cell epitopes recognized in humans, non-human
primates, rodents, and other animal species. The current focus of the I
EDB is the curation of epitopes from infectious diseases, but epitopes
relevant in allergy and autoimmune diseases will also be included in the
near future. In this workshop, we will demonstrate how to extract data
from the IEDB using multiple query methods. For example, we will
illustrate how to extract epitopes given a particular pathogen, protein
or host, how to query for T cell epitopes restricted by a certain MHC
allele and how to query for linear and discontinuous B cell epitopes. In
addition to the queries, we will demonstrate online epitope prediction
and analysis tools including MHC binding predictions, population
coverage, epitope conservancy analysis, and sequence homology mapping
tools available at the IEDB. You're invited to bring your own laptop
(not required) to follow along and we will assist you on how to
customize searches and analyses for your purposes.
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